restriction enzyme cloning with xbai (New England Biolabs)
99
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New England Biolabs
restriction enzyme cloning with xbai
Restriction Enzyme Cloning With Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+enzyme+cloning+with+xbai/XbaI/pm41714755-141-33-38
Average 99 stars, based on 6783 article reviews
Restriction Enzyme Cloning With Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+enzyme+cloning+with+xbai/XbaI/pm41714755-141-33-38
Average 99 stars, based on 6783 article reviews
restriction enzyme cloning with xbai - by Bioz Stars,
2026-09
99/100 stars
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Expressing:Article Title: Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cells. Article Snippet: Generation of stable cell lines HEK293T cells were transfected with pLentiCRISPRv229 with EpCAM guide RNA30 (Genscript, Piscataway, NJ, USA), lentiviral packaging plasmid psPAX2 (courtesy of Didier Trono, Addgene #12260, Watertown, MA, USA), and envelope plasmid PMD2.G (courtesy of Didier Trono, Addgene #12259) using Lipofectamine 3000 (Thermo Fisher Scientific #L3000-008) according to the manufacturer’s instructions. .. To generate the EpCAM overexpressing (OV) and mutEpCAM expressing plasmids, custom genes were produced by Thermo Fisher Scientific GeneArt and inserted into pLentiCMV-blast31 (courtesy of Eric Campeau & Paul Kaufman, Addgene #17486) using Produced:Article Title: Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cells. Article Snippet: Generation of stable cell lines HEK293T cells were transfected with pLentiCRISPRv229 with EpCAM guide RNA30 (Genscript, Piscataway, NJ, USA), lentiviral packaging plasmid psPAX2 (courtesy of Didier Trono, Addgene #12260, Watertown, MA, USA), and envelope plasmid PMD2.G (courtesy of Didier Trono, Addgene #12259) using Lipofectamine 3000 (Thermo Fisher Scientific #L3000-008) according to the manufacturer’s instructions. .. To generate the EpCAM overexpressing (OV) and mutEpCAM expressing plasmids, custom genes were produced by Thermo Fisher Scientific GeneArt and inserted into pLentiCMV-blast31 (courtesy of Eric Campeau & Paul Kaufman, Addgene #17486) using Cloning:Article Title: Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cells. Article Snippet: Generation of stable cell lines HEK293T cells were transfected with pLentiCRISPRv229 with EpCAM guide RNA30 (Genscript, Piscataway, NJ, USA), lentiviral packaging plasmid psPAX2 (courtesy of Didier Trono, Addgene #12260, Watertown, MA, USA), and envelope plasmid PMD2.G (courtesy of Didier Trono, Addgene #12259) using Lipofectamine 3000 (Thermo Fisher Scientific #L3000-008) according to the manufacturer’s instructions. .. To generate the EpCAM overexpressing (OV) and mutEpCAM expressing plasmids, custom genes were produced by Thermo Fisher Scientific GeneArt and inserted into pLentiCMV-blast31 (courtesy of Eric Campeau & Paul Kaufman, Addgene #17486) using other:Article Title: Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cells Article Snippet: After puromycin selection and a round of cell sorting with an Aria IIu Cell Sorter the Johns Hopkins Ross Flow Cytometry Core Facility using an anti-EpCAM antibody conjugated to Alexa Fluor 647 (1:200 dilution, Santa Cruz Biotechnology #sc25308-AF647, Dallas, TX, USA), the EpCAM knockout (KO) remained unstable, showing EpCAM expression to slowly increase again over time, indicating a mixed EpCAM KO and WT cell population. |